Can Nucleotides Spontaneously Form DNA in Aqueous Solution?

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Discussion Overview

The discussion centers on the potential for nucleotides to spontaneously form DNA in an aqueous solution, exploring the necessary components and conditions for such a process to occur.

Discussion Character

  • Exploratory, Technical explanation, Debate/contested

Main Points Raised

  • One participant questions the feasibility of DNA formation from nucleotides alone, suggesting that a sugar-phosphate backbone is also necessary.
  • Another participant clarifies that the backbone must consist of alternating sugars and phosphates, with each sugar linked to a nucleotide base.
  • A different viewpoint argues that even with the correct components, spontaneous assembly may not yield recognizable DNA, referencing historical experiments that required additional biological materials.
  • One participant expresses appreciation for a historical reference related to RNA assembly, indicating a connection to the broader context of molecular biology.

Areas of Agreement / Disagreement

Participants do not reach a consensus on whether nucleotides can spontaneously form DNA, with multiple competing views regarding the necessity of additional components and the conditions required for assembly.

Contextual Notes

The discussion highlights the importance of specific components (sugars, phosphates) in the formation of DNA and references historical experiments that may influence current understanding, but does not resolve the complexities involved.

Loren Booda
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Given high and approximately equal concentrations of nucleotides Adenine, Cytosine, Guanine and Thymine in aqueous solution, how readily could a simple DNA molecule form there?
 
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You'd need the backbone sugars, too.

- Warren
 
To be precise, it's a sugar-phosphate backbone (alternating), where every sugar has a nucleotide base attached.
 
Alright, make the solution a balance of appropriate sugars, phosphates and nucleotides.
 
I don't think this would auto-assemble into anything you would recognize as DNA. Nirenberg's famous auto-assembly of poly-uracyl RNA used a starter "sap", the crushed cytoplasm from a bacterium, along with the test tube of uracyl. See http://history.nih.gov/exhibits/nirenberg/HS4_polyU.htm
 
selfAdjoint,

In my work (for a mental health line), I refer people to NIH quite often. Thanks for the link to that wonderful story, of which I was unaware until now.
 

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