How do you calculate the concentration of nucleotides from DNA?

[nancy189]

Hi,

I was wondering if given the concentration of DNA, how does one calculate the concentration of nucleotides? Are they the same thing?

Thanks.
Nancy

 

[Ygggdrasil]

The concentration of nucleotides would just be the concentration of DNA multiplied by the number of nucleotides per molecule of DNA. For example a 1nM solution of a 100bp piece of double-stranded DNA would have a nucleotide concentration of 200nM.