I'm referring to paper chromatography. And by upper portion, I mean the upper area where the solvent column on the paper strip is moving forward, and by lower portion I mean the lower area on the paper strip where the solvent column appears to have stopped.DrClaude said:This question is hard to understand. What kind of chromatography are you referring to? And what do you mean by "upper portion" and "lower portion"?
Chromatography is a laboratory technique used to separate and analyze mixtures of substances based on their physical and chemical properties.
Chromatography works by passing a mixture through a medium, such as a solid or liquid, that allows the components of the mixture to separate based on their relative affinities for the medium and the mobile phase they are traveling through.
Kinetic energy in chromatography refers to the energy of motion of the molecules in the mixture as they move through the medium and separate. Potential energy, on the other hand, refers to the energy stored in the bonds between the molecules and the medium.
The difference in kinetic energy between the components of a mixture affects their relative speed and movement through the medium. Components with higher kinetic energy will travel faster and elute first, while components with lower kinetic energy will elute later.
The difference in kinetic energy can be influenced by factors such as the type of medium used, the composition of the mobile phase, the temperature, and the strength of the intermolecular forces between the components and the medium.