Citric Acid Cycle -matching carbons

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SUMMARY

The discussion focuses on tracing carbon atoms from glucose to pyruvate in the Citric Acid Cycle. Each glucose molecule, containing six carbons, is converted into two pyruvate molecules, each with three carbons. The cleavage of fructose-1,6-bisphosphate by aldolase is identified as a key step for understanding the distribution of these carbons. Participants suggest numbering the carbons at each step of the pathway for easier tracking.

PREREQUISITES
  • Understanding of the Citric Acid Cycle
  • Knowledge of glycolysis and its intermediates
  • Familiarity with the role of aldolase in carbohydrate metabolism
  • Basic skills in biochemical pathway analysis
NEXT STEPS
  • Study the process of glycolysis in detail, focusing on fructose-1,6-bisphosphate cleavage
  • Learn how to diagram the Citric Acid Cycle and label carbon atoms
  • Explore the role of enzymes in metabolic pathways, particularly aldolase
  • Investigate carbon tracing techniques in metabolic studies
USEFUL FOR

Students of biochemistry, educators teaching metabolic pathways, and researchers interested in carbohydrate metabolism will benefit from this discussion.

jenzao
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Citric Acid Cycle --matching carbons

Which glucose carbons are pyruvate carbons from? I've been trying to get this for hours. please help.. Also how do you trace carbons along the ciric acid cycle?
 
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Each pyruvate is a 3 carbon molecule and glucose is a 6 carbon molecule. Therefore, half of the carbons in a glucose molecule go to each of the two pyruvate molecules. The step where the molecule is cut in half I believe is the step where fructose-1,6-bisphosphate is cleaved by aldolase so you should be able to see which carbons go where by analyzing the result of that step. If you want to track the carbons, the easiest thing to do is to just number them on each step of the pathway.
 

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