If you trap 1 electron in a dot between two leads ----o---- it will cause a repulsive potential barrier for another electron that could have been trapped there -to high, so it cannot be trapped -Its blocked by coulomb repulsion. To overcome this you lower the potential close to the dot with a gate electrode, and now the electron 2 could be trapped also. But the 3:d electron is blocked from the 2 electrons to be trapped, so you must lower the gate potential again, and so on.