To find the eigenvalues of a positive definite matrix, the characteristic polynomial can be used to identify its roots, which are the eigenvalues and should be positive. For large matrices, methods like the power iteration and the Lanczos algorithm are recommended, although caution is advised with the latter due to potential numerical issues. For a matrix size of 100x100, various software packages such as MATLAB, Maple, and Mathematica can efficiently compute all eigenvalues. QR decomposition is also a viable method for this size, with computations typically taking about one second on modern PCs. Overall, utilizing existing algorithms and software is the most practical approach for finding eigenvalues in larger matrices.