How Do You Calculate Molar Mass from Concentration Data?

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To calculate concentration from absorbance data, use the line of best fit or linear regression to relate absorbance to concentration. Once concentration is determined, convert it to molar mass by calculating the number of moles from the concentration and using the mass of the sample. The relationship between absorbance and concentration is established through Beer's Law, which is fundamental in spectrophotometry. The discussion emphasizes the importance of accurate calibration using a blank solution. Understanding these concepts is crucial for completing the lab successfully.
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Hello! I'm completing a lab for chemistry and would like some help on the last bit figuring out concentration then converting to molar mass. I need to make a data chart with a line of best fit on it with the dependent/independent variables. I understand that concentration is on the X-axis and the absorption is on the Y-axis. I used a spectrophotometer to track the different absorbency values of the different dilutions we made. I calibrated the machine using a "Blank" which was water in a test tube. I made a test solution, then made different dilutions using a stock solution provided to my class.

The observation chart that was produced looked like:

{Solutions}{Absorbency}{Wavelength (Blue)}​

Blank (H2O) 0.00 470nm
Test Sol'n 5g solute/25mL H2O 1.41 470nm
Stock Sol'n 0.83 470nm
0.40 mol/L sol'n 12mL S.S./3mL H2O 0.70 470nm
0.30 mol/L sol'n 9mL S.S./6mL H2O 0.58 470nm
0.20 mol/L sol'n 6mL S.S./9mL H2O 0.40 470nm
0.10 mol/L sol'n 3mL S.S./12mL H2O 0.21 470nm​

**Sol'n = solution**
**S.S. = stock solution**
**[ ] = concentration**

1. How do you find concentration?
2. How do you convert concentration to molar mass?
 
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3riefs said:
1. How do you find concentration?

Read it from the line of the best fit. Or, if you use linear regression, calculate concentration from absorbance. That's the same.

2. How do you convert concentration to molar mass?

Knowing concentration you will be able to calculate number of moles, knowing number of moles and mass of the sample you will be able to get molar mass.
 

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