I did a search using terms
stress strain nylon rope, and found a document titled Review of Synthetic Fiber Ropes:
https://apps.dtic.mil/sti/pdfs/ADA084622.pdf. It has a good discussion of what happens when a rope is subjected to repetitive loadings. That discussion is consistent with the observations of
@B0B about ropes breaking after a number of uses. That document is highly suggested reading for anybody who finds this thread interesting.
The following stress strain curves are from that document:
View attachment 322629
Note that nylon does not have a linear stress strain curve. That means that the linear spring equations, such as ##Energy = 0.5 Kx^2## do not apply. It is necessary to integrate by measuring the area under the stress strain curve.
Since the OP wants to know the energy storage capability of one full length of rope, his best approach is to measure the deflection of a full length of rope. Measuring the strain of a short piece, and using that to calculate the deflection of the entire length, is not only a waste of effort, but also introduces errors. The above plot shows the need for a series of measurements. The resulting data plot would have the horizontal axis in feet and the vertical axis in pounds. It would apply to one specific rope. The results would be directly useful for the energy calculations desired by the OP.