Fluorescence and Raman spectroscopy

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SUMMARY

The discussion centers on the challenges faced in recording Raman spectra using a mercury lamp through carbon tetrachloride (CCl4) at wavelengths of 435.8 nm and 404.7 nm. The user, W, reports consistent results at 435.8 nm but inconsistent frequency shifts and the absence of anti-Stokes lines at 404.7 nm. Claude suggests that the interference of fluorescence may be the cause of these unexpected results, emphasizing that fluorescence can overshadow the Raman signal, leading to measurement difficulties.

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wakko101
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I'm currently working on a lab that is exploring the Raman effect. One of the suggested exercises was to record the raman spectrum (from a mercury lamp through CCl4) around the rayleigh peaks at 435.8 nm and 404.7 nm. For the former, my results were fairly consistent with what I expected in that the frequency shifts were mostly on the ball. However, in the latter wavelength, 404.7, not only do my frequency shifts fall off the mark, but I only see stokes lines and no anti-stokes lines. My thoughts were that this might be due to the interference of fluorescence, but I'm not too familiar with this phenomenon.

Does anyone have any ideas as to why I am getting the results that I have? Any help would be most appreciated.

Cheers,
W. =)
 
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Fluorescence typically means no measurement at all, since it is so much stronger than the Raman signal.

Are your Stokes lines actually cosmic ray peaks?

Claude.
 

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