Undergrad Measuring Thickness and Refractive Index with Fourier Domain OCT

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SUMMARY

This discussion focuses on measuring the thickness and refractive index of a sample using Fourier Domain Optical Coherence Tomography (OCT). The user has successfully calculated the thickness of a glue layer on a glass microscope slide but seeks guidance on determining the refractive index of the glue. Key considerations include the transparency and color of the glue, which can affect the measurement process.

PREREQUISITES
  • Understanding of Fourier Domain Optical Coherence Tomography (OCT)
  • Knowledge of optical properties, specifically refractive index
  • Familiarity with sample preparation techniques for microscopy
  • Basic principles of light interaction with transparent materials
NEXT STEPS
  • Research methods for measuring refractive index using Fourier Domain OCT
  • Explore the impact of sample transparency on OCT measurements
  • Learn about optical coherence tomography calibration techniques
  • Investigate the properties of various glue materials used in microscopy
USEFUL FOR

Researchers and technicians in optical engineering, materials science, and microscopy who are involved in sample characterization and optical measurements.

Mkyb242
Hi, I would like to know how one can simultaneously measure the thickness and refractive index of a sample using Fourier Domain OCT. I have a glue layer on a glass microscope slide and I've calculated the thickness of the glue layer, but I'm unsure of how to the refractive index of the glue. Any help would be greatly appreciated.
 
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Is the glue transparent? Does it have any color?
 
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