General operational theory of fluorescence is

In summary, fluorescence is the process in which a molecule absorbs and then emits light, creating a characteristic glow. However, not all molecules are equally efficient at this process, as some factors such as chemical structure and environmental conditions can affect the ability to emit light. This is known as fluorescence quenching.
  • #1
OnePound
3
0
Hello,

I think the general operational theory of fluorescence is understood - a molecule accepts a photon, its electrons are excited from the ground state to a higher energy state, and, as they return to the ground state, they emit EM waves which we see as light. My question is what makes some molecules or compounds better at doing this than others? Surely all molecules should be able to do this, as all molecules have electrons that can be excited?

Many thanks,
OnePound
 
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  • #2


As explained in the Wikipedia article on Fluorescence, there are a number of ways in which the atom can deexcite without emitting a photon. See especially Wiki's article on "Quenching (fluorescence)"
 

What is fluorescence?

Fluorescence is a physical phenomenon in which molecules absorb light at a specific wavelength and emit light at a longer wavelength. This emission of light is called fluorescence and can be seen as a glow or color change.

How does fluorescence work?

Fluorescence works by the absorption of photons, or particles of light, by a molecule. This absorption causes the molecule to become excited and move to a higher energy state. The molecule then releases this excess energy by emitting light at a longer wavelength, resulting in fluorescence.

What is the general operational theory of fluorescence?

The general operational theory of fluorescence explains the process of fluorescence in terms of the interaction between light and matter. It involves the absorption of light, movement to a higher energy state, and subsequent emission of light at a longer wavelength.

What are the applications of fluorescence in science?

Fluorescence has many applications in science, including in biological and medical research, environmental monitoring, and materials science. It is commonly used in techniques such as fluorescence microscopy, flow cytometry, and fluorescence spectroscopy.

How is fluorescence used in research and technology?

Fluorescence is widely used in research and technology due to its sensitivity, selectivity, and non-invasive nature. It is used to study the structure and function of biomolecules, detect and measure various substances, and develop new materials and technologies. It also has applications in fields such as forensics and diagnostics.

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