Genetics double recombination counted twice in terms of RF

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SUMMARY

This discussion focuses on the calculation of recombination frequency (RF) in a trihybrid cross, specifically addressing the treatment of double recombinants. In a typical trihybrid cross, 6 out of 8 possible genotypes are recombinant, and the RF is determined by the number of crossover events divided by the total progeny. The participants debate the rationale behind counting double recombinants as contributing twice to the RF, emphasizing the need for clarity in visualizing genetic distances between loci. Accurate mapping of genetic distances requires careful consideration of crossover events, particularly in cases involving double recombinants.

PREREQUISITES
  • Understanding of genetic recombination and parental genotypes
  • Familiarity with trihybrid crosses and genotype proportions
  • Knowledge of recombination frequency calculation methods
  • Basic concepts of genetic mapping and centimorgans
NEXT STEPS
  • Research the calculation of recombination frequency in genetic mapping
  • Study the implications of double recombinants on genetic distance measurement
  • Explore the concept of centimorgans and their application in genetics
  • Learn about advanced genetic mapping techniques and tools
USEFUL FOR

Geneticists, biology students, and researchers involved in genetic mapping and analysis of recombination events will benefit from this discussion.

despues357
When mapping out re-combinations of a crossed trihybrid you have to account for the proportion of the offspring who do not share parental genotypes.

Of the 8 possible genotypes stemming from this mating, 6 will be recombinant and two will be of parental genotypes.

Your idea is to Map out the distance between each Subject allele that crossed over by counting their recombinant frequency value as map units (centrimorgans).I'm not sure why you would count double recombinants as twice their frequency simply because there were two cross overs. more like, I can't visualize how that could be interpreted as doubling the perceived distance between these two loci...
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To figure out the distance between two genes, you need to figure out the recombination frequency, which is the number of crossover events that happen between the two genes divided by the total number of progeny tested. In the double recombinant, there are two crossover events happening between the V and CV genes. Therefore, you need to take this into account when calculating the recombination frequency.
 

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