MHB Help with solution concentration

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To create a 10% bleach solution for DNA contamination removal, a calculation is needed based on the active chlorine concentration of 4.47g per 100ml, which indicates a 4.47% bleach solution. Mixing standard household bleach, typically 3-6%, won't achieve the desired 10% concentration. The discussion suggests that to make a 10% solution, one would need to combine one part bleach with nine parts water, but this approach won't work with the given bleach concentration. Therefore, achieving a 10% solution requires a more concentrated bleach source or a different method. Accurate measurements and understanding of the bleach's active ingredient concentration are crucial for effective solution preparation.
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Hi I'm looking for some help that I think is easy but just can't get my head round.
So I need to make a solution with a concentration of 10% bleach to remove DNA contamination from equipment. So if I had 100ml of water I'd need 10ml of bleach to make a 10% solution. But it is the active chlorine in bleach that removes DNA so I'm trying to work out a calculation of how many ml of bleach I'd need if the active chlorine concentration within the bleach is 4.47g per 100ml. Can anyone advise how I work out the ml of beach needed to get 10% solution.
Thanks.
 
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Noclue said:
Hi I'm looking for some help that I think is easy but just can't get my head round.
So I need to make a solution with a concentration of 10% bleach to remove DNA contamination from equipment. So if I had 100ml of water I'd need 10ml of bleach to make a 10% solution. But it is the active chlorine in bleach that removes DNA so I'm trying to work out a calculation of how many ml of bleach I'd need if the active chlorine concentration within the bleach is 4.47g per 100ml. Can anyone advise how I work out the ml of beach needed to get 10% solution.
Thanks.

Hi Noclue, welcome to MHB! ;)

If I'm not mistaken, a 4.47 g of active chlorine bleach per 100 ml of water, is a 4.47% bleach solution.
A typical household bleach is a 3–6% solution in water.
We can't get from there to 10% with just mixing.
 
It would help to see the actual text that you are trying to interpret. I would guess that "bleach" refers to standard household bleach and that they are saying to make a solution of 1 volume of household bleach added to 9 volumes of water.
 
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