Help with solution concentration

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SUMMARY

The discussion centers on creating a 10% bleach solution for DNA contamination removal, specifically calculating the required volume of bleach given its active chlorine concentration of 4.47g per 100ml. A participant clarifies that the 4.47g concentration indicates a 4.47% bleach solution, which is insufficient for achieving a 10% concentration through simple mixing. The consensus is that standard household bleach, typically ranging from 3% to 6%, cannot be diluted to reach a 10% concentration without using a more concentrated bleach solution.

PREREQUISITES
  • Understanding of solution concentration and dilution principles
  • Knowledge of active ingredients in household bleach
  • Basic mathematical skills for concentration calculations
  • Familiarity with laboratory safety protocols when handling bleach
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  • Research how to prepare concentrated bleach solutions for laboratory use
  • Learn about the chemical properties of sodium hypochlorite in bleach
  • Study dilution calculations and their applications in laboratory settings
  • Explore alternative methods for DNA contamination removal
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This discussion is beneficial for laboratory technicians, researchers in molecular biology, and anyone involved in DNA analysis or contamination control who requires precise solution preparation techniques.

Noclue1
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Hi I'm looking for some help that I think is easy but just can't get my head round.
So I need to make a solution with a concentration of 10% bleach to remove DNA contamination from equipment. So if I had 100ml of water I'd need 10ml of bleach to make a 10% solution. But it is the active chlorine in bleach that removes DNA so I'm trying to work out a calculation of how many ml of bleach I'd need if the active chlorine concentration within the bleach is 4.47g per 100ml. Can anyone advise how I work out the ml of beach needed to get 10% solution.
Thanks.
 
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Noclue said:
Hi I'm looking for some help that I think is easy but just can't get my head round.
So I need to make a solution with a concentration of 10% bleach to remove DNA contamination from equipment. So if I had 100ml of water I'd need 10ml of bleach to make a 10% solution. But it is the active chlorine in bleach that removes DNA so I'm trying to work out a calculation of how many ml of bleach I'd need if the active chlorine concentration within the bleach is 4.47g per 100ml. Can anyone advise how I work out the ml of beach needed to get 10% solution.
Thanks.

Hi Noclue, welcome to MHB! ;)

If I'm not mistaken, a 4.47 g of active chlorine bleach per 100 ml of water, is a 4.47% bleach solution.
A typical household bleach is a 3–6% solution in water.
We can't get from there to 10% with just mixing.
 
It would help to see the actual text that you are trying to interpret. I would guess that "bleach" refers to standard household bleach and that they are saying to make a solution of 1 volume of household bleach added to 9 volumes of water.
 

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