Can the Hendersen Hasselbach equation be used for higher pH values?

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SUMMARY

The Henderson-Hasselbalch equation can be effectively utilized for buffer solutions at pH values higher than the pKa of the buffering agent. In this discussion, the focus is on preparing a 1.5 M Tris-HCl buffer at pH 8.8 using Tris base and HCl. The consensus confirms that Tris serves as a suitable buffering agent, allowing for precise pH adjustments with HCl and NaOH. Therefore, researchers can confidently apply the Henderson-Hasselbalch equation for their buffer preparations at elevated pH levels.

PREREQUISITES
  • Understanding of the Henderson-Hasselbalch equation
  • Knowledge of buffer preparation techniques
  • Familiarity with Tris base and its properties
  • Basic skills in pH measurement and adjustment
NEXT STEPS
  • Research the specific pKa values of Tris to understand its buffering range
  • Learn about the preparation and adjustment of Tris buffers
  • Explore the effects of ionic strength on buffer capacity
  • Investigate alternative buffering agents for high pH applications
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Chemists, laboratory researchers, and students involved in buffer preparation and pH adjustment in biochemical experiments.

rockind78
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I am starting to make the various buffers for my research, but I am a bit rusty with my chemistry. I want to know if hendersen hasselbach equation can be used if the desired pH is higher than the pKA. Specifically, I want to make 1.5 M Tris-HCl buffer (pH 8.8) utilizing Tris base and HCl. I can work the math just fine in the HH, but I am a little unusure about the flexibility in its application. Any help would be greatly appreciated. Thank you.

Dustan
 
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Here they say it works for pH 8.0 http://ntri.tamuk.edu/fplc/prep.html

As I understand it Tris is the buffering agent, and with HCl and NaOH you can set the pH to the desired point.
 
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