How Do You Calculate Total Absorption of a Sample from Its Absorbance Spectrum?

  • Context: Graduate 
  • Thread starter Thread starter Rositta
  • Start date Start date
  • Tags Tags
    Absorption
Click For Summary
SUMMARY

The correct method to calculate the total absorption of a sample from its absorbance spectrum involves performing a numerical integration over the entire wavelength range, using the spectral intensity of the light source as a weighting factor. This approach is more accurate than simply averaging the absorbance data, which can yield inconsistent results. The integration method ensures that all relevant wavelengths are considered, providing a comprehensive measure of total absorption.

PREREQUISITES
  • Understanding of absorbance spectra and their components
  • Familiarity with numerical integration techniques
  • Knowledge of spectral intensity and its role in absorption calculations
  • Basic principles of light-matter interaction
NEXT STEPS
  • Research numerical integration methods in spectroscopy
  • Learn about the impact of spectral intensity on absorbance calculations
  • Explore software tools for spectral analysis, such as MATLAB or Python libraries
  • Study case studies on absorption measurements in various materials
USEFUL FOR

Researchers in chemistry and physics, analytical chemists, and anyone involved in spectroscopic analysis of materials.

Rositta
Messages
13
Reaction score
0
Hi, does anyone know how to calculate the total absorptin of a sample from its labdam-absorbance spectrum?
I found a paper where the author find it as the average of the data, another one numerically integrate the spectrum over the whole lambda range.. but the two methods give different results... what's the corecct way?
 
Science news on Phys.org
Average of what data?

An integration over the wavelength spectrum, with the spectral intensity of the light source as weight, should give the correst result.
 

Similar threads

Graduate Subtract Radiated from Background Spectrum to Analyze Absorption Spectrum
  • · Replies 12 ·
Replies
12
Views
3K
Undergrad How do emissivity and absorptivity affect thermal imaging results?"
  • · Replies 3 ·
Replies
3
Views
3K
Graduate Q: Calculate average length of photon path: emit->absorb
  • · Replies 17 ·
Replies
17
Views
4K
Calculation of absorption spectra
  • · Replies 3 ·
Replies
3
Views
3K
Graduate Correlated-k method (absorption of radiation)
  • · Replies 3 ·
Replies
3
Views
2K
How Do You Calculate Molar Absorptivity?
  • · Replies 11 ·
Replies
11
Views
5K
Graduate Trying to meaningfully deconvolute IR absorption spectra
  • · Replies 1 ·
Replies
1
Views
3K
Graduate How Do Scientists Determine the Composition of a Star's Core?
  • · Replies 3 ·
Replies
3
Views
2K
Graduate How to compute phase gradient from Snell's law?
  • · Replies 1 ·
Replies
1
Views
3K
Graduate Determining MDA for the Dual Label Counting method with the LSC
  • · Replies 0 ·
Replies
0
Views
2K