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How long will my gas tank will last?

  1. Jan 29, 2012 #1
    How long will my gas tank last?

    Dear All,

    I've a question for you that's driving me a little bit crazy. I'm trying to set up low oxygen tension cell culture chambers to grow my cells. Here is a picture:

    attachment.php?attachmentid=43256&stc=1&d=1327867120.jpg

    The chamber is cylindrical shape and has a volume of 1.2 liters, it's at room temperature and room air. I purchased a 50 liters tank at 150 bar of 2% oxigen, 5% CO2 and 93% N2. My protocols says to connect the tank with a 1/4 inches tube to the chamber and let the gas flow through the chamber at 2 psi for 2 minutes. Then I seal the two holes of the chamber and the air mix inside will be 2% O2, 5% CO2 and 93% N2.

    I know the protocol is correct, so I don't care to reduce the flow time. I'm just wondering how many chambers I can prepare with a 50 liters, 150 bar tank.

    Of course I'm looking for an approximate solution of my problem...it's just for cost estimation.

    Thanks!
     

    Attached Files:

    Last edited: Jan 29, 2012
  2. jcsd
  3. Jan 31, 2012 #2
    It's impossible to calculate unless you know the flow rate in liters per minute. Get a rotameter and attach it to your tube and measure the flow rate at 2 psi.
     
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