The discussion focuses on calculating the gradient of the separation vector from a fixed point to a variable point in Cartesian coordinates. The separation vector, denoted as c', is defined, and its length c is expressed as the square root of the sum of squared differences in coordinates. The key formula derived is Gradient(1/c) = -c'(hat)/c^2, where "hat" indicates the unit vector in the direction of c'. Participants clarify the differentiation process using the chain rule, emphasizing that primed terms remain constant during differentiation. The conversation concludes with a brief explanation of the term "hat" in this context.