The discussion centers on methods for safely destroying a deadly virus in laboratory settings. Key techniques include the use of viricidal agents such as 70% ethanol, isopropanol, sodium hypochlorite, and autoclaving, which employs high temperature and pressure to inactivate viruses. Participants emphasize the importance of using chemical virucides in conjunction with autoclaving for thorough disinfection, especially when handling dangerous virus strains. The conversation also touches on the safety protocols required for working with biosafety level 2 (BSL2) and level 4 (BSL4) pathogens.
PREREQUISITESLaboratory technicians, virologists, biosafety professionals, and anyone involved in infectious disease research and laboratory safety practices.
misskitty said:That would make sense. Do you know the maximum temperature that plastic can withstand before melting?
Specific Plastic Considerations
Polypropylene, polymethylpentene, polypropylene copolymer, TEFZEL ETFE, TEFLON FEP, and PFA may be autoclaved repeatedly at 121°C, 15 psig. Cycles should be at least 15 minutes to ensure sterility.
Polycarbonate products are autoclavable. They must be thoroughly rinsed before autoclaving because detergent residues cause crazing and spotting. Autoclaving cycles should be limited to 20 minutes at 121°C. PC shows some loss of mechanical strength after repeated autoclaving and therefore may not function well under high-stress applications, such as centrifugation. Our PC vacuum chambers are considered "not autoclavable" for this reason.
Do not use strong alkaline detergents on polycarbonate. Do not use boiler steam containing alkaline chemical additives that may attack the plastic and cause the item to fail.
Acetal products are autoclavable at recommended settings. Proper ventilation is required as acetal will emit formaldehyde odor during autoclaving. The following statement complies with the California Safe Drinking Water and Toxic Enforcement Act of 1986: "WARNING: Upon autoclaving, this product may release formaldehyde, a chemical known to the State of California as a carcinogen."
Polysulfone products are autoclavable. They are somewhat weakened by repeated autoclaving, although less than polycarbonate. If autoclaved repeatedly, polysulfone products will eventually fail under high-stress applications, such as high-speed centrifugation.
NALGENE PVC Tubing can be autoclaved, but ethylene oxide or chemical disinfectant is preferred. If you autoclave it, follow these guidelines:
Clean and rinse tubing thoroughly, including final rinse with distilled or deionized water. Coil tubing loosely and keep ends open. Wrap in muslin or linen; tape or tie loosely. Place on a nonmetallic tray in the autoclave so wrapped tubing is not touching wall or rack of autoclave. Do not stack anything on the tubing. Use 15 minute cycle at 121°C, 15 psig. Restore clarity of tubing by drying approximately 2 hours at a temperature no higher than 75°C.
NALGENE Silicone Tubing can be autoclaved for 30 minutes at 121C, 15 psig in muslin cloth or sterilizing paper.
Labware made of the following plastics is not autoclavable under any conditions: polystyrene, polyvinyl chloride (except PVC tubing), styrene acrylonitrile, acrylic, low-density and high-density polyethylene and polyurethane.
misskitty said:What other kinds of viruscides are there?