SUMMARY
To create a 100 µM stock solution from 26.6 nMoles of dried primer, one must convert the primer amount to µMoles, resulting in 0.0266 µMoles. The appropriate formula to use is C1V1 = C2V2, where C1 is the initial concentration (0.0266 µM), C2 is the desired concentration (100 µM), and V2 is the final volume. By rearranging the formula, the required volume of water to add can be calculated to achieve the desired concentration.
PREREQUISITES
- Understanding of molarity and concentration calculations
- Familiarity with the C1V1 = C2V2 dilution equation
- Knowledge of unit conversions between nMoles and µMoles
- Basic principles of solution preparation
NEXT STEPS
- Practice using the C1V1 = C2V2 formula with different concentrations
- Learn about unit conversions between different mole measurements
- Explore methods for preparing stock solutions in laboratory settings
- Investigate the implications of concentration on chemical reactions
USEFUL FOR
Students in chemistry or biochemistry, laboratory technicians, and anyone involved in solution preparation and concentration calculations.