How to determine the volume of water to add to a dried primer

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SUMMARY

To create a 100 µM stock solution from 26.6 nMoles of dried primer, one must convert the primer amount to µMoles, resulting in 0.0266 µMoles. The appropriate formula to use is C1V1 = C2V2, where C1 is the initial concentration (0.0266 µM), C2 is the desired concentration (100 µM), and V2 is the final volume. By rearranging the formula, the required volume of water to add can be calculated to achieve the desired concentration.

PREREQUISITES
  • Understanding of molarity and concentration calculations
  • Familiarity with the C1V1 = C2V2 dilution equation
  • Knowledge of unit conversions between nMoles and µMoles
  • Basic principles of solution preparation
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  • Practice using the C1V1 = C2V2 formula with different concentrations
  • Learn about unit conversions between different mole measurements
  • Explore methods for preparing stock solutions in laboratory settings
  • Investigate the implications of concentration on chemical reactions
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Students in chemistry or biochemistry, laboratory technicians, and anyone involved in solution preparation and concentration calculations.

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Homework Statement


A volume of highly purified water is added to dried primer to create 100uM stock solution. How many ul of water needs to be added to 26.6nMoles of dried primer in order to create uM stock solution? Hint: You may need to convert primer value from nMoles to uMoles.

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The Attempt at a Solution


I know that 26.6nMoles is 0.0266uMoles. But I am unsure of what to do next. Must I use C1V1=C2V2? Or does 10 x 26.6 = 266ul work?
 
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ver_mathstats said:
Or does 10 x 26.6 = 266ul work?
Work for what?
ver_mathstats said:
I know that 26.6nMoles is 0.0266uMoles
OK, so if you dissolved it in 1L the concentration would be 0.0266 µM. You want 100 µM. This is where you apply C1V1 = C2V2.
 
mjc123 said:
Work for what?

OK, so if you dissolved it in 1L the concentration would be 0.0266 µM. You want 100 µM. This is where you apply C1V1 = C2V2.
Oh okay, I think the formula is c=n/v. Thank you.
 

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