How to prepare PLGA nanoparticles?

Thread starter Kunal Aggarwal
Start date Sep 11, 2017
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Chemical engineering Nano technology Prepare

In summary, PLGA (poly(lactic-co-glycolic acid)) is a commonly used biodegradable and biocompatible polymer in the preparation of nanoparticles. Different methods, such as emulsion/solvent evaporation, nanoprecipitation, and salting-out, can be used to prepare PLGA nanoparticles, and the choice depends on the desired properties and the drug being encapsulated. The appropriate PLGA to drug ratio can vary and is determined through experimental optimization and characterization studies. The size and morphology of PLGA nanoparticles can be affected by factors such as the method of preparation, molecular weight and composition of the polymer, and the presence of stabilizers or surfactants. Various techniques, including dynamic light scattering, scanning electron

Sep 11, 2017

Kunal Aggarwal

What is the protocol used to prepare poly (lactic-co-glycolic acid) (PLGA) nanoparticle? What are the different methods of producing it?

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Sep 17, 2017

Kunal Aggarwal

Which nanoparticles can used to deliver tuberculosis drugs in the body and what is the protocol of making such nanoparticles (such as PLGA)?

1. What is PLGA and why is it used to prepare nanoparticles?

PLGA stands for poly(lactic-co-glycolic acid), which is a biodegradable and biocompatible polymer commonly used in the preparation of nanoparticles. It is widely used in the pharmaceutical industry due to its ability to encapsulate and protect drugs, as well as its controlled release properties.

2. What are the different methods for preparing PLGA nanoparticles?

There are several methods for preparing PLGA nanoparticles, including emulsion/solvent evaporation, nanoprecipitation, and salting-out. Each method has its own advantages and disadvantages, and the choice of method depends on the desired properties of the nanoparticles and the drug being encapsulated.

3. How do I determine the appropriate PLGA to drug ratio for my nanoparticles?

The PLGA to drug ratio can vary depending on the specific drug and the method of preparation. It is important to consider the solubility of the drug in the polymer, as well as the desired drug loading and release rate. Experimental optimization and characterization studies are typically performed to determine the optimal ratio for a particular drug.

4. What are the factors that affect the size and morphology of PLGA nanoparticles?

The size and morphology of PLGA nanoparticles can be influenced by several factors, including the method of preparation, the molecular weight and composition of the polymer, the drug to polymer ratio, and the presence of stabilizers or surfactants. It is important to carefully control these factors to achieve nanoparticles with desired properties.

5. How can I characterize and analyze my PLGA nanoparticles?

There are various techniques for characterizing and analyzing PLGA nanoparticles, including dynamic light scattering, scanning electron microscopy, and Fourier-transform infrared spectroscopy. These techniques can provide information on the size, shape, surface charge, and drug encapsulation efficiency of the nanoparticles, which are important for understanding their properties and potential applications.