The coordinates of the end points will be different with different frames - not surprising when you consider that a "frame" is just a convention used to assign these coordinates. However, all the geometric facts about the line, anything that you can say about it without reference to coordinates (it does or does not pass through a given event, it does or does not intersect another line or a surface, ...) will of course hold in all frames. Less obviously, but at least as important, the length (properly termed a "spacetime interval") of the line will be the same in all frames, even though the coordinates of the endpoints are different.
The spacetime interval ##\Delta{s}## between two events ##(t_1,x_1,y_1,z_1)## and ##(t_2,x_2,y_2,z_2)## is given by ##\Delta{s}^2={-c^2\Delta{t}^2+\Delta{x}^2+\Delta{y}^2+\Delta{z}^2}## where ##\Delta{x}## is defined as ##x_2-x_1## and likewise for the other three coordinates, and it comes out the same in all frames even though the individual coordinate values are different.