Chemistry Mechanism for Solid-Phase Nucleic Acid Deprotection and Cleavage

[physicisttobe]

Homework Statement

Deprotection and Delaware reaction

Relevant Equations

…

Hi everyone!

I can‘t solve this following task. Can you explain me how I have to draw the mechanism?

Task: Solid-phase nucleic acid synthesis: deprotection and cleavage of carrier with 2'-O-TOM protected suitable phosphoramidite building block synt. dinucleotide of 5'-UG.

I started with deprotection, but how should I continue? The task is a little bit strang. But I hope you understand it.

 

[TeethWhitener]

I agree that the task is very vague. Can you list out the steps in the phosphoramidite method?

 

[physicisttobe]

Yes, I can do it. But I think the task is about the cleavage and deprotection of a specific functional group. Therefore, I draw the cleavage mechanism. And how schould I draw the deprotection reaction?
I also used the Tom method here. But which base must I use for the second nucleotide? I simply wrote base-acyl because I did not know which base to use. I also dit not know which protecting group to use for the second nucleotide. Therefore, I wrote OPG. Which potecting group should I use for the second nucleotide ? Which would be appropriate?