Purpose of Helium Source in HPLC: Degassing & More

  • Thread starter Thread starter peteza
  • Start date Start date
  • Tags Tags
    Helium Source
Click For Summary
SUMMARY

The regulated helium source in High-Performance Liquid Chromatography (HPLC) serves two primary functions: it degasses solvents and creates a protective blanket of helium over the solvent reservoirs. This blanket prevents atmospheric air from re-dissolving into the solvents, ensuring the integrity and consistency of the chromatographic process. Utilizing helium enhances the overall performance and reliability of HPLC systems.

PREREQUISITES
  • Understanding of High-Performance Liquid Chromatography (HPLC) principles
  • Knowledge of solvent properties and their behavior in chromatography
  • Familiarity with gas chromatography techniques
  • Basic concepts of degassing methods in analytical chemistry
NEXT STEPS
  • Research the impact of degassing on HPLC performance
  • Explore different types of gas sources used in chromatography
  • Learn about the effects of dissolved gases on solvent behavior
  • Investigate advanced HPLC techniques for solvent management
USEFUL FOR

Analytical chemists, laboratory technicians, and researchers involved in chromatography who seek to optimize HPLC performance and solvent management strategies.

peteza
Messages
3
Reaction score
0
What's the purpose of the regulated helium source going into the solvent reservoirs in HPLC? Is it just to degas the solvents? Does it have any other use?
 
Chemistry news on Phys.org
It degasses the solvents and provides a blanket of Helium over the reservoir to prevent air from dissolving back into the solvent.
 

Similar threads

Need advice and hints for working with helium
  • · Replies 14 ·
Replies
14
Views
2K
Please help me understand the LC/MS procedure in the lab
  • · Replies 2 ·
Replies
2
Views
1K
Cleaning a small batch of HDPE flakes obtained from motor oil containers
  • · Replies 2 ·
Replies
2
Views
4K
Suitable condenser for dichloromethane solvent
  • · Replies 1 ·
Replies
1
Views
2K
Which HPLC Column is Best for Separating Enantiomer Proteins?
  • · Replies 3 ·
Replies
3
Views
4K
Undergrad Which Ion Source for a Helium Particle Accelerator?
  • · Replies 3 ·
Replies
3
Views
3K
Helium compound produced, closing the last gap
  • · Replies 6 ·
Replies
6
Views
3K
Theoretical ξr of a molecule from the absolute configuration
  • · Replies 2 ·
Replies
2
Views
6K
How does the 'Helium' Network work?
  • · Replies 10 ·
Replies
10
Views
3K
Carbon dioxide in water, analysis of dissolved species HPLC?
  • · Replies 11 ·
Replies
11
Views
5K