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Raihn
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A typical protein contains 16.2% nitrogen (14.00674 mg/mmol). A 0.500 mL aliquote of protein solution was digested, and the liberated NH3 was distilled into 10.00mL of 0.02140 M HCL. The unreacted HCl required 3.26mL of 0.0198 M NaOH for complete titration. Find the concentration of protein (mg protein/ mL) in the original sample.
I'm not entirely sure on where to start first. Should I convert the HCl and NaOH into moles? Or? Not entirely sure of what to do first. If someone can give me a hint, most appreciated.
If converting them to moles is the first step, where do I go from there to find the concentration of the protein?
Thank you in advanced.
I'm not entirely sure on where to start first. Should I convert the HCl and NaOH into moles? Or? Not entirely sure of what to do first. If someone can give me a hint, most appreciated.
If converting them to moles is the first step, where do I go from there to find the concentration of the protein?
Thank you in advanced.