Questions Regarding Methods of Gene Cloning

[Sunwoo Bae]

TL;DR

Comparing traditional restriction digestion based cloning with Gibson assembly cloning

I have been told that the traditional restriction digestion cloning method, where you use restriction enzyme to produce sticky ends in the gene of interest and the plasmid vector, is vulnerable to contamination. The lecturer proceeds to conclude that Gibson Assembly Cloning is therefore a cheaper and safer way of gene cloning. Why is the traditional digestion cloning method vulnerable to contamination, and why is Gibson Assembly cloning not vulnerable to contamination?

Thank you!

 

[Ygggdrasil]

That's probably something you should ask the lecturer. In particular, what is mean by "contamination"? I've used both techniques and I'm not entirely sure of what the lecturer is trying to say from the information you've given.