Restriction enzyme reads a DNA strand containing nucleotide base pairs

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SUMMARY

A restriction enzyme reads a DNA strand and cuts it after identifying a specific sequence of 6 nucleotide base pairs, AAGCCT. The probability of the enzyme correctly reading each base is 1/4, leading to a calculation of (1/4)^6 for the likelihood of this sequence occurring. The discussion highlights the need for clarification regarding the context of the DNA strand and whether the sequence can be read from both ends. Without additional information about the DNA strand's length or structure, determining the average length of a cut strand remains ambiguous.

PREREQUISITES
  • Understanding of DNA structure and nucleotide base pairs
  • Knowledge of restriction enzymes and their function
  • Basic probability concepts, particularly regarding independent events
  • Familiarity with the concept of reading sequences in both directions
NEXT STEPS
  • Research the function and mechanisms of restriction enzymes in molecular biology
  • Learn about DNA sequence analysis and the significance of nucleotide patterns
  • Study probability calculations in biological contexts, particularly for sequences
  • Explore the implications of reading DNA sequences from both ends and its biological relevance
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Biologists, geneticists, and students studying molecular biology who are interested in understanding the mechanics of DNA manipulation and the probabilistic nature of nucleotide sequences.

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Homework Statement



I know this is a biology question but this is the most busy forum so I decided to put it here:

I'll make this simple. A restriction enzyme reads a DNA strand containing nucleotide base pairs. It cuts the strand after it reads a sequence of 6 base pairs - AAGCCT. Of course the probability of the enzyme reading the correct base is 1/4. And it needs 6 of them.

So what is the average length of a cut strand? I'm thinking you do (1/4)^6 and this will give you something.. but what?

Thanks for your help in advance...



Homework Equations





The Attempt at a Solution

 
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Please put the questions on the correct forum. Our mods will just have to move it and theyre over worked as it is.:smile:
Sorry can't help you with Biology. Thats also a problem, the biology people don't come here to answer bio questions.
 
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I'm more of a physics and math person than a bio person, but two questions: Is this DNA that's competely random? (from the math point of view), and the bigger question: Does it matter which end you're coming from? i.e. if you had a strand that was CCAAGGTCCGAATAGC, although AAGCCT doesn't appear from left to right, it does appear when going from right to left. (My bit of knowledge makes me believe it can work from both ends)
 
Okay, forget making it "simple." Can you give your problem exactly as it was given to you? What is the context? The length of a cut strand is the distance between the restriction sites, so there isn't enough information provided about what it is you're cutting to know that.

Or, are you trying to find the probability of that exact 6 nucleotide sequence occurring more than once in a random sequence? Without being given any information about the length of the DNA strand, you have to assume they're talking about the distance between two restriction sites and not from a restriction site to the end of the DNA.

So, please come back with some clarification of what the problem is, or we can't do much to help.
 

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