- #1
sameeralord
- 662
- 3
Hello everyone,
[PLAIN]http://users.rcn.com/jkimball.ma.ultranet/BiologyPages/R/RestrictionEnzymes.gif
1. Restriction enzymes cut only sugar phospahte bonds right. So in the formation of sticky bonds for example in BamHl, how does the hydrogen bonds between the molecules break. When sugar phophate bonds on each strand break, does it pull on the free side dissociating the hydrogen bonds. Can these pieces stick back easily again or do they need a ligase?
2. Can restriction enzymes cut single stranded DNA?
3. Also how do these detect specific sequences of DNA. Do they have some complementary area that binds with the specific region.
Thanks
[PLAIN]http://users.rcn.com/jkimball.ma.ultranet/BiologyPages/R/RestrictionEnzymes.gif
1. Restriction enzymes cut only sugar phospahte bonds right. So in the formation of sticky bonds for example in BamHl, how does the hydrogen bonds between the molecules break. When sugar phophate bonds on each strand break, does it pull on the free side dissociating the hydrogen bonds. Can these pieces stick back easily again or do they need a ligase?
2. Can restriction enzymes cut single stranded DNA?
3. Also how do these detect specific sequences of DNA. Do they have some complementary area that binds with the specific region.
Thanks
Last edited by a moderator: