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Separation Through chromatography

  1. Oct 13, 2014 #1
    Dear all,
    We all know that chromatography is a separation technique. In paper or TLC,the mixture is differentiated according to their relative affinities with the solvent on the paper or glass plate. How do we really separate the mixture. Kindly, help me how to extract the components of mixture from paper or TLC.
    Thanks
     
  2. jcsd
  3. Oct 13, 2014 #2

    Borek

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    Staff: Mentor

    TLC is not typically used for extraction, rather to check what is present in the solution/how is the reaction going. If you want to separate substances for further use, use column chromatography.
     
  4. Oct 13, 2014 #3
    You mean Paper and TLC..?
     
  5. Oct 13, 2014 #4

    Borek

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    Staff: Mentor

    Yes.

    In case small amount is enough, you can do the chromatography (TLC or paper), cut the stain and extract the substance in small amount of solvent. But in general that's not what TLC and paper chromatography is for.
     
  6. Oct 17, 2014 #5

    epenguin

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    I often used to use TLC to purify nucleoside triphosphates , (DEAE-cellulose with NaCl eluant) - it was pretty simple, only small quantities were needed and you very easily see the bands of the triphosphates and of impurities by UV illumination. Just came back to me that before chromatogrphing the sample, we prepared the DEAE-cellulose plates putting the bottom of the plate in water or dilute salt and this pushed some stuff out of the DEAE which you could see as a dark band that moved with the front, then we dried them. Don't knows if many people would use it nowadays though.
     
  7. Mar 21, 2016 #6
    I agree with Borek. Column chromatography is the way forward for typical purification processes. Let me know if you need help with that. :)

    Cheers
     
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