Separation Through chromatography

In summary, the conversation discusses the use of chromatography as a separation technique, specifically in paper or TLC. It is mentioned that TLC is not typically used for extraction, but rather to check the presence of substances in a solution. For further use and purification, column chromatography is recommended. The use of TLC for purification is also mentioned, with a specific example of using it to purify nucleoside triphosphates. It is suggested to use column chromatography for typical purification processes.
  • #1
Deadevil
21
0
Dear all,
We all know that chromatography is a separation technique. In paper or TLC,the mixture is differentiated according to their relative affinities with the solvent on the paper or glass plate. How do we really separate the mixture. Kindly, help me how to extract the components of mixture from paper or TLC.
Thanks
 
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  • #2
TLC is not typically used for extraction, rather to check what is present in the solution/how is the reaction going. If you want to separate substances for further use, use column chromatography.
 
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Likes SimOpera
  • #3
You mean Paper and TLC..?
 
  • #4
Yes.

In case small amount is enough, you can do the chromatography (TLC or paper), cut the stain and extract the substance in small amount of solvent. But in general that's not what TLC and paper chromatography is for.
 
  • #5
Borek said:
Yes.

In case small amount is enough, you can do the chromatography (TLC or paper), cut the stain and extract the substance in small amount of solvent. But in general that's not what TLC and paper chromatography is for.

I often used to use TLC to purify nucleoside triphosphates , (DEAE-cellulose with NaCl eluant) - it was pretty simple, only small quantities were needed and you very easily see the bands of the triphosphates and of impurities by UV illumination. Just came back to me that before chromatogrphing the sample, we prepared the DEAE-cellulose plates putting the bottom of the plate in water or dilute salt and this pushed some stuff out of the DEAE which you could see as a dark band that moved with the front, then we dried them. Don't knows if many people would use it nowadays though.
 
  • #6
I agree with Borek. Column chromatography is the way forward for typical purification processes. Let me know if you need help with that. :)

Cheers
 

1. What is chromatography?

Chromatography is a technique used to separate and analyze mixtures of compounds based on their different physical and chemical properties.

2. What are the different types of chromatography?

There are several types of chromatography, including gas chromatography, liquid chromatography, and thin-layer chromatography. Each type uses different methods and equipment for separation.

3. How does chromatography work?

In chromatography, a mixture is separated as it moves through a medium, such as a column or a thin layer of material. The compounds in the mixture will interact differently with the medium, causing them to separate and form distinct bands or spots.

4. What is the purpose of using chromatography?

The main purpose of chromatography is to separate and analyze the components of a mixture. This can be useful in identifying unknown substances, determining the purity of a sample, and studying the properties of different compounds.

5. What are some common applications of chromatography?

Chromatography has a wide range of applications in various industries, including pharmaceuticals, food and beverage, environmental testing, and forensics. It is also commonly used in research and development to study the composition and behavior of different substances.

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