Tandem MS: What Does Time in MRM Graph Refer To?

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SUMMARY

The discussion focuses on the interpretation of the "time" axis in the Multiple Reaction Monitoring (MRM) graph produced by tandem mass spectrometry (MS). In MRM, ions are selected in MS1, fragmented, and then analyzed in MS2, resulting in a graph that plots intensity against time rather than m/z. The "time" refers to the retention time of ions on the chromatography column, indicating when specific m/z ratios are detected. Multiple graphs can be generated for different m/z ratios selected by the quadrupole, providing a comprehensive analysis of the sample.

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Homework Statement


There are four scan modes for tandem MS: Product ion, Precursor ion, Neutral loss scan mode and MRM.
e.g. In MRM, the result of graph is Intensity versus time, but NOT versus m/z.
What does the "time" in the graph refers to?

Homework Equations

The Attempt at a Solution


For MRM, ions are selected in MS1 and fragmented in collision cell. The daughter ions then selected in MS2 and go to the detector.
The result of graph should be intensity versus m/z. But it is against time. Does it means it the selected m/z in MS2 undergo LC after that? Or the time is the detection time?
 
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The time is the retention time on the column. You should have multiple graphs corresponding to the different m/z ratios selected by your quadrupole.
 

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