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rockind78
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"expressed at a basal level"; used in reference to expression of the the human metallothionein gene.
Any thoughts would be most welcome. Thanks!
Any thoughts would be most welcome. Thanks!
Originally posted by Monique
So rockind, were they talking about RNA or protein expression levels? Basal levels would be the level when expression is not induced :)
Originally posted by rockind78
OK. Found it...it was in the Lewin text, I have been using Alberts as a secondary source, they are both excellent. It looks to me like it is in the context of protein expression, but why don't you be the judge:
"The metallothionein (MT) gene provides an example of how a single gene may be regulated by many different circuits. The metallothionein protein protects the cell against excess concetrations of heavy metals, by binding the metal and removing it from the cell. The gene is expressed at the basal level, but is induced to greater levelsof expression by hevy metal ions (such as cadmium)or by glucocorticoids. The control region provides several different kinds of regulatory elements."
Expressed at a basal level refers to the level of gene expression that is consistently and constantly present in an organism's cells without any external stimulation or stress. It is the baseline level of gene activity that is necessary for the normal functioning of the cell.
Basal gene expression can be influenced by a variety of factors, including the type of cell, the stage of development, and the surrounding environment. Genetic mutations and epigenetic changes can also affect basal gene expression.
Basal gene expression can be measured through techniques such as RNA sequencing, microarrays, and qPCR. These methods allow scientists to quantify the amount of mRNA present in a cell and determine the level of gene expression.
Basal gene expression is essential for the proper functioning of cells and maintaining homeostasis in an organism. It also serves as a starting point for changes in gene expression in response to external stimuli or during developmental processes.
Basal gene expression can be altered through mutations, epigenetic modifications, and changes in the cellular environment. External factors such as stress, nutrition, and hormones can also influence basal gene expression.