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What kind of errors would be produced by excess taq in PCR?

  1. Feb 22, 2010 #1

    Wek

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    What kind of errors would be produced by "excess" taq in PCR?

    Not sure if this belong in the Homework section. Put it here since it's not homework.

    What kind of errors will I get by adding 6.75µL taq pol to +50µL of cDNA? The master mix will be added to the cDNA resulting in an excess of taq pol. Only 2 and 4µL of the taq+cDNA mixture will be used for each reaction not the entire +50µL.
     
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  3. Feb 27, 2010 #2

    Monique

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    Re: What kind of errors would be produced by "excess" taq in PCR?

    There is not information here to answer your question. As a rule you never add more than 10% of enzyme to your reaction, this is because your enzyme is stored in glycerol and that this is inhibitory to the reaction.
     
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