What Solvent Can Be Used to Dissolve Collagen?

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SUMMARY

The discussion centers on effective solvents for dissolving collagen, specifically highlighting the use of hot water at approximately 100°C, which denatures collagen, and citrate buffer at a pH of 3.4 for solubilizing undenatured collagen. The referenced study, published in the Journal of Applied Polymer Science by Leo D. Kahn and Lee P. Witnauer, details an automated apparatus for collagen solubilization, achieving a maximum of 6.95% solubilization of calfskin corium in citrate buffer at pH 3.44. This information is crucial for researchers and practitioners working with collagen in various applications.

PREREQUISITES
  • Understanding of collagen structure and properties
  • Familiarity with pH and buffer solutions, specifically citrate buffer
  • Knowledge of thermal denaturation processes
  • Basic laboratory techniques for solubilization
NEXT STEPS
  • Research the effects of temperature on collagen denaturation
  • Explore the use of citrate buffer in biochemical applications
  • Investigate automated solubilization techniques for collagen
  • Review literature on collagen solubilization methods and their efficiencies
USEFUL FOR

Researchers, biochemists, and professionals in the fields of tissue engineering and food science who are involved in collagen extraction and application will benefit from this discussion.

zhengw
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In acetic acid?? but I find it is difficult, which solvent I could use? thank you very much
 
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hot water works fine. Water at about 100C will solubilize it. It will denature.

Undenatured collagen can be solubilized with citrate buffer at about pH of 3.4.
See here:

http://www3.interscience.wiley.com/cgi-bin/abstract/104021707/ABSTRACT?CRETRY=1&SRETRY=0
 
Thank you very much.
Unfortunately, I can't open the link which you give. Is that a paper?? can you tell me the name of the title and the author.
Thank you again
 
Journal of Applied Polymer Science
Volume 13, Issue 1, Date: January 1969, Pages: 141-148
Leo D. Kahn, Lee P. Witnauer

Abstract
An automated collagen solubilizing apparatus that maintains constant electrolytic conditions during solubilization is described. It was used in solubilization of 6.95% of a calfskin corium as monomeric collagen in citrate buffer at pH 3.44 and ionic strength of 0.44 and a temperature of 8°C. It is shown that this is the limit of solubilization for undenatured collagen in this buffer.
 
I have got the paper
Thank you very much
 

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