Why Are 488nm LED Options Hard to Find for Cell Sorting?

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SUMMARY

Finding 488nm LED options for fluorescence activated cell sorting (FACS) is challenging due to limited availability and the high cost of traditional argon ion lasers. Users are seeking affordable alternatives, with suggestions including various online retailers such as Thorlabs, LD Selection, LaserGlow, and Coherent. The discussion emphasizes the importance of using sensitive detectors to minimize illumination intensity for live cell applications, ensuring that the light source does not damage the cultures.

PREREQUISITES
  • Understanding of fluorescence activated cell sorting (FACS)
  • Knowledge of light sources, specifically 488nm LEDs
  • Familiarity with sensitive detection methods for live cell applications
  • Basic knowledge of laser technology and its alternatives
NEXT STEPS
  • Research affordable 488nm LED options from Thorlabs and LaserGlow
  • Explore the specifications and applications of sensitive detectors for live cell imaging
  • Investigate filtering techniques to isolate desired wavelengths in fluorescence applications
  • Learn about the differences between LED and laser light sources in biological applications
USEFUL FOR

Researchers, biologists, and laboratory technicians involved in fluorescence activated cell sorting and those seeking cost-effective alternatives to traditional laser systems.

SupaVillain
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Why can't I find any LED's at 488nm? I am trying to find a cheaper light source than the traditional argon ion laser for a possible fluorescence activated cell sorter. See link below for schematic. Does anybody know a good retail site for lights in this spectrum I can't find good results with google.

http://thebigone.stanford.edu/papers/Fu%20nature%20america.pdf
 
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SupaVillain said:
Why can't I find any LED's at 488nm? I am trying to find a cheaper light source than the traditional argon ion laser for a possible fluorescence activated cell sorter. See link below for schematic. Does anybody know a good retail site for lights in this spectrum I can't find good results with google.

http://thebigone.stanford.edu/papers/Fu%20nature%20america.pdf
How well focused does it have to be? That is, if it has a significant amount at 488 is it OK for it to have quite a bit in the surrounding region of the spectrum and if so, how far away from 488 is OK? There are inexpensive LED sources that show a range that includes 488.
 
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I have no idea, i mean i bet i can filter out the unwanted parts of the spectrum anyways but really I think for biofluorescence it shouldn't take much power. When i think of a laser and high power, all that does is burn whatever it's pointed at. Not trying to burn the cultures. I would much rather try out the cheap version than spend a bunch of money on something i don't really need. Can you link me what you see at 488?
 
Hm ... I was thinking UV spectrum at 388, not visible at 488. I can't see why you would want 488 if what you are looking for is fluorescence. To get 488 just buy a $4 "black light" bulb at a hardware store (but I don't think you'll get any fluorescence off of what you shine it on; at least I know that's true of wood)