SUMMARY
Objects smaller than the wavelength of visible light cannot be observed using optical microscopes due to the limitations imposed by diffraction and scattering. The resolution of optical microscopes is approximately 200 nm, determined by the wavelength of light and the numerical aperture (NA). Techniques such as electron microscopy and scattering microscopy have been developed to visualize smaller objects, as they do not rely on visible light. The phenomenon of Rayleigh scattering explains why small particles do not reflect photons effectively, making them invisible to standard optical methods.
PREREQUISITES
- Understanding of optical microscopy principles
- Familiarity with diffraction and its effects on imaging
- Knowledge of Rayleigh scattering and its implications
- Basic concepts of numerical aperture (NA) in microscopy
NEXT STEPS
- Research the principles of electron microscopy and its applications
- Study the diffraction patterns of circular apertures and Airy disks
- Explore advanced techniques in scattering microscopy
- Learn about the limitations and capabilities of ultraviolet microscopy
USEFUL FOR
Physicists, optical engineers, and researchers in materials science seeking to understand the limitations of optical microscopy and explore advanced imaging techniques for small-scale objects.