Would exonic primers affect gDNA amplification?

  • Thread starter Wek
  • Start date
  • #1
Wek
18
0
Will a primer designed for an exonic sequence anneal and sucessfully amplify a gDNA strand? I'm having trouble amplifying gDNA using that primer. cDNA with that primer amplifies perfectly. I have no problem amplifying gDNA using another primer that anneals to intronic sequences.
 

Answers and Replies

  • #2
44
0
I can't think of a reason why any given primer should work only for cDNA. Maybe the reason why you're having trouble with the genomic DNA amplification has to do with other possible annealing sites on the genomic DNA? I had this problem before. If you have upstream/downstream genetic information, maybe you should try a nested PCR?
 

Related Threads on Would exonic primers affect gDNA amplification?

  • Last Post
Replies
1
Views
6K
Replies
3
Views
2K
Replies
2
Views
822
  • Last Post
Replies
2
Views
2K
  • Last Post
Replies
2
Views
2K
Replies
10
Views
1K
  • Last Post
Replies
1
Views
3K
Replies
1
Views
841
Replies
4
Views
7K
Replies
5
Views
2K
Top