yes, I agree that there are easier ways to express Km but the lab isn't asking for that determination sadly. however, the problem has been resolved. Km can be determined by combining equations
Kcat = K2K3/(K2+K3) ≈ K3 (if K2>>K3)
Km = K3K/(K2+K3)
I did a practical and am now trying to do the data analysis of it. the prac was the alpha-chymotrypsin catalyzed hydrolysis of 4-nitrophenyl trimethylacetate.
I have values for X, Y and B and the lab manual has asked "Using the approximated, simplified expressions for X (equation 2), Y (equation...