Recent content by miraiw

  1. miraiw

    Preservation of protein structure in electroblotting

    Rethinking it, I guess I might have asked why the structure is preserved in the electrophoresis... So denaturing preserves the primary structure. The probing is done on linear epitopes then? I think my real question was what kinds of forces act on and within proteins and polymers generally such...
  2. miraiw

    OpenWorm: The Secrets of C. elegans Through Open Science

    The campaign was a success! Thanks to all who contributed to our funding -- your support won't be in vain. Also to any who looked into OpenWorm and chose to support the project in other ways :)
  3. miraiw

    Preservation of protein structure in electroblotting

    Hello, I am reading about the Western blot technique, and I am looking for clarification of the transfer step described in the wikipedia article and in this video walkthrough. It is not clear to me why the proteins should keep their shape needed for the probing step rather than breaking or...
  4. miraiw

    OpenWorm: The Secrets of C. elegans Through Open Science

    Hi PF members, I'm writing code for OpenWorm this summer. Our goal is to simulate the entire worm C. elegans for the purposes of testing biophysical models at the level of single cells, of systems of cells, and of interaction with the worm's environment. Our current focus is on the nervous...
  5. miraiw

    Could fluorescence quenching be used for making 3D images?

    Are you responding to the post as a whole, or to only the idea of "presenting dynamic 3D forms in high resolution"? I think I could have made my question clearer. Please look at this video here: The demonstration in the video is exactly the kind of thing I'm interested in. Probably you would...
  6. miraiw

    Could fluorescence quenching be used for making 3D images?

    What I have in mind is a programmable medium for presenting dynamic 3D forms in high resolution. I don't think I know enough to measure or expirement with this, but the image in the article seemed suggestive of a system where an array of lasers would be directed towards a volume of quinine, the...
  7. miraiw

    Could fluorescence quenching be used for making 3D images?

    Looking at the image from this wikipedia article, it looks like there's a selective fluorescing of the quinine dependent on the presence of chrolide ions in the solution. If the concentration of chloride ions in a solution could be controlled into a particular shape, shining a violet laser on...
  8. miraiw

    Identities of nested set algebraic expressions

    I restated the problem in terms of propositions like $$x \in A_{1} \wedge x \in A_{2}$$ and considered that whatever was in the ellipsis would either depend on the innermost expression or not and if it did would either have a value opposite the innermost or the same as. From there I just...
  9. miraiw

    Identities of nested set algebraic expressions

    Are there any useful identities for simplifying an expression of the form: $$((\ldots((x_{1} *_{1} x_{2}) *_{2} x_{3}) \ldots) *_{n - 1} x_{n})$$ Where each $$*_{i}$$ is one of $$\cap, \cup$$ and $$x_1 \ldots x_n$$ are sets? I believe I found two; though I haven't proved them, I think they...
  10. miraiw

    Change of coordinates resulting in scale

    Homework Statement Consider q: ℝ^{2}→ℝ q(x,y) = \left[x\ y\right] S \left[\stackrel{x}{y}\right] Show that with the change of coordinates \left[\stackrel{x}{y}\right] = Q\left[\stackrel{x'}{y'}\right] that q(x,y) =\lambda_{1}x'^{2} + \lambda_{2}y'^{2} Homework Equations S =...
  11. miraiw

    Is my Leyden jar experiment properly designed?

    Thanks for the replies. It's at least working now.
  12. miraiw

    Is my Leyden jar experiment properly designed?

    What do you mean, exactly?
  13. miraiw

    Is my Leyden jar experiment properly designed?

    http://imagebin.org/139123 So I should put foil on the outside? I can see how that would work better, but it should work with my hand.
  14. miraiw

    Is my Leyden jar experiment properly designed?

    I'm attempting to make a Leyden jar by taking a plastic cup, covering the inside with aluminium foil, and then filling the cup with salt water. I have a spring sticking through the centre that conducts through the inner foil. Judging from the Wikipedia article on Leyden jars I can use my hand...
  15. miraiw

    Is time travel possible and does it mean we have no free will?

    The question of free will is a self-interested thing. Note that people typically want to know if they don't have it, whether they can do as they please or they are silently being guided by a big man in the sky. In that case, it's not a question for physics but for sociology and psychology--it's...
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