"I Put a Spell on You" has been covered dozens of times, perhaps most famously by Nina Simone on the 1965 album I Put A Spell On You, but also by performers such as Natacha Atlas, Audience, Batmobile, Creedence Clearwater Revival (who performed it at Woodstock), Them, Sonique, Arthur Brown, The Animals, Leslie West, Tim Curry, Alan Price (former keyboardist with The Animals), Joe Cocker, Nick Cave, Ray Charles, Bryan Ferry, Marilyn Manson, Eels, Julien Doré, Kim Nalley, Buddy Guy (featuring Carlos Santana), and many others. There is a live performance by Pete Townshend on his Deep End Live! album (with David Gilmour).
Most of the covers treat the song seriously; few attempt to duplicate Hawkins's bravura performance.
Also, it has been sampled in songs by Notorious B.I.G. (“Kick In The Door”) and by LL Cool J (“LL Cool J”).
In films, it has been performed (with different lyrics) by Bette Midler in Disney's Halloween movie, Hocus Pocus, of which this version has been used as eixt music for the HalloWishes Halloween-themed fireworks show at Mickey's Not-So-Scary Halloween Party in Walt Disney World's Magic Kingdom theme park; while the original version has also been covered by Diamanda Galás, which was featured in Oliver Stone's Natural Born Killers. Marilyn Manson's version was featured in David Lynch's Lost Highway as well as television ads for M. Night Shyamalan's The Sixth Sense. The Creedence Clearwater Revival version was used in Rebecca Miller's The Ballad of Jack and Rose. It has been also played in an episode of The Simpsons (I'm Spelling as Fast as I Can) and an episode of The PJ's. It was featured in the movie Stranger Than Paradise. Hawkins performed the song on film in A Rage in Harlem.
The Hawkins version has even become a standard accompaniment for ice skaters, including Michelle Kwan, Alexei Urmanov and the team of Elizabeth Punsalan and Jerod Swallow.