Cholesterol Extraction using Soxhlet Method

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The discussion focuses on using the Soxhlet Method to extract cholesterol from egg yolk with dichloromethane. The experiment involves crushing half-boiled egg yolk and adding 80 cm³ of the solvent. A key question arises about quantifying cholesterol absorption at a wavelength of 550 nm. The participant expresses confusion about how to approach the question, recognizing that it relates to measuring concentration using a visible light spectrometer. Clarification on the methodology for quantification is sought.
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Homework Statement



During an experiment using Soxhlet Method to extract cholesterol from egg yolk, also using dicloromethane as a reagent, we crush half boiled egg yolk and put 80 cm3 of dicloromethane.

Homework Equations



Then comes a question which is assuming the maximum of absorption of the reagent-cholestrol is 550 nm, sugest a way to quantify it.

The Attempt at a Solution



I don't understand what should I answer here
 
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As worded it doesn't make much sense. Obviously it is about measuring concentrating using visible light spectrometer.
 

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