To differentiate between alpha particles and helium in an enclosed space, a mass spectrometer or residual gas analyzer (RGA) can be employed, as they effectively detect helium by ionizing it for measurement. Measuring pressure can also indicate helium levels if it is the only gas present. For experiments involving alpha decay, a silicon detector can be utilized to identify charged alpha particles. Accurate results depend on having a calibrated measurement device or standard for comparison. Understanding the principles of vacuum technology is essential, and resources like "A User's Guide to Vacuum Technology" by John F. O'Hanlon can provide valuable insights.