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jojoba
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Automated DNA synthesis was performed using CPG solid support (with the first
base already attached) with 10 nmol (=10-9 mol) capacity (meaning you can recover
up to 10 nmol of synthetic DNA after the synthesis). Answer the following questions.
You synthesized the following sequence 5’-GACCG(N)10AAAAA3’ (N represents
a degenerate base representing an equal mix of the four bases). Assuming a
100% coupling efficiency (just for the sake of argument… you never get 100% in
real life), what is the maximum number of distinct DNA sequences in the final
DNA mixture?
The class doesn't have an assigned textbook so I don't have a source to refer to. Does anyone have any ideas how I should start this problem?
base already attached) with 10 nmol (=10-9 mol) capacity (meaning you can recover
up to 10 nmol of synthetic DNA after the synthesis). Answer the following questions.
You synthesized the following sequence 5’-GACCG(N)10AAAAA3’ (N represents
a degenerate base representing an equal mix of the four bases). Assuming a
100% coupling efficiency (just for the sake of argument… you never get 100% in
real life), what is the maximum number of distinct DNA sequences in the final
DNA mixture?
The class doesn't have an assigned textbook so I don't have a source to refer to. Does anyone have any ideas how I should start this problem?