Rate of Glucose uptake by yeast cells

[xXAznGurlXx]

You are required to plan an investigation to find out the rate of glucose uptake by yeast cells varies with glucose concentration

material resources
- 10% glucose solution
- disilled water
-yeast suspension
- Benedicts reagent
I have done the introduction, apparatus required, but I am unsure on what quantity i should use to make various glucose concentrations and i don;t know how to go about with the method :/
I was wondering if u guys be able to help me, thanksss

 

[Kushal]

you have to dilute the glucose solution. you start with 10%, then 5%, 1%, 0.5%. it depends on how many readings you need.

to dilute you need to add a specific amount of water to the solution. you have to calculate that amount.

 

[Blueshift5]

Sure, I would be happy to help you plan your investigation. The first step in planning an experiment is to clearly define your research question, which in this case is: "How does the rate of glucose uptake by yeast cells vary with glucose concentration?"

Next, you will need to determine the range of glucose concentrations you want to test. It is important to have a range of concentrations to accurately determine the relationship between glucose concentration and uptake rate. I would recommend starting with a low concentration (such as 1%) and increasing by increments of 1% until you reach a high concentration (such as 10%). This will give you a range of 1-10% glucose concentrations to test.

In terms of quantities, you will need to make sure that each glucose concentration has the same volume and concentration of yeast suspension. For example, if you decide to use 10 mL of each glucose concentration, you will need to make sure that each 10 mL contains the same amount of yeast suspension. This will ensure that the only variable being tested is the glucose concentration.

As for the method, here is a suggested procedure:

1. Label 10 test tubes with the different glucose concentrations (1%, 2%, 3%, etc.).
2. Measure out 10 mL of the 10% glucose solution and pour it into the first test tube.
3. Using a pipette, add 10 mL of distilled water to the first test tube to create a 1% glucose solution.
4. Repeat this process for each test tube, adding 10 mL of distilled water to each test tube to create the desired concentration.
5. Next, add the same amount of yeast suspension to each test tube (for example, 1 mL).
6. Mix the contents of each test tube well and allow the yeast cells to incubate for a set amount of time (such as 30 minutes).
7. After the incubation period, add Benedicts reagent to each test tube and observe the color change. The Benedicts reagent will indicate the amount of glucose that has been taken up by the yeast cells.
8. Record the results and repeat the experiment at least three times to ensure accuracy.
9. Use the results to create a graph showing the relationship between glucose concentration and uptake rate.

It is important to note that there may be other factors that can affect the rate of glucose uptake by yeast cells, such as temperature and pH. Therefore, it is important to control these variables as