Size resolution limitation on Dynamic Light Scattering

In summary, Dynamic Light Scattering (DLS) is a technique used in analytical chemistry to measure the size distribution of particles in a solution by analyzing the fluctuations in the intensity of scattered light caused by Brownian motion. The size resolution limitation for DLS is typically around 1 nanometer, which can affect the accuracy of measurements. However, there are techniques that can be applied to improve the size resolution limitation, such as using multiple scattering angles, a lower wavelength of laser light, and advanced data analysis algorithms. Other techniques for measuring the size of nanoparticles include electron microscopy, atomic force microscopy, and nanoparticle tracking analysis, each with their own advantages and limitations. The choice of technique depends on the specific needs of the experiment or analysis.
  • #1
JD_PM
1,131
158
I have been looking online and it is stated to be 1nm, but these are posts around 6 years old:

https://www.azonano.com/article.aspx?ArticleID=3662

https://www.researchgate.net/post/Whats_the_measurement_limit_of_dynamic_light_scattering

I am wondering if the limiting resolution has improved on DLS or it has stayed the same.
 
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  • #3
Greg Bernhardt said:
@ZapperZ do you know?

No, sorry. This is an experimental technique that I've never used. All the light scattering experiments that I've done are for spectroscopy, not imaging or spatial resolution.

Zz.
 

1. What is the principle behind Dynamic Light Scattering?

The principle behind Dynamic Light Scattering is that particles in a solution will scatter light, and the intensity of this scattered light is related to the size of the particles. By analyzing the fluctuations in the scattered light intensity over time, the size of the particles can be determined.

2. What is the size range that can be measured using Dynamic Light Scattering?

The size range that can be measured using Dynamic Light Scattering is typically between 1 nanometer to 1 micrometer. However, this range can vary depending on the instrument and the properties of the particles being measured.

3. How does the sample concentration affect the size resolution in Dynamic Light Scattering?

The sample concentration can affect the size resolution in Dynamic Light Scattering because at high concentrations, the particles may interact with each other and form aggregates, leading to inaccurate size measurements. It is recommended to dilute the sample to a concentration where the particles are well-separated for optimal size resolution.

4. Are there any limitations to the accuracy of size measurements using Dynamic Light Scattering?

Yes, there are limitations to the accuracy of size measurements using Dynamic Light Scattering. These limitations include the assumption that the particles are spherical and have a uniform refractive index, as well as potential errors from instrument settings and data analysis methods. It is important to carefully consider these factors when interpreting size data from Dynamic Light Scattering.

5. Can Dynamic Light Scattering be used for all types of particles?

No, Dynamic Light Scattering may not be suitable for all types of particles. For example, particles that are highly polydisperse or non-spherical may produce inaccurate size measurements. In addition, certain types of particles, such as large aggregates or opaque particles, may not scatter enough light to be detected by the instrument. It is important to consider the properties of the particles when deciding if Dynamic Light Scattering is an appropriate size measurement technique.

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