Recent content by fcs

Hitachi Solutions just launched a free web application for 4PL and 5PL curve-fitting: http://www.readerfit.com" Just like MasterPlex ReaderFit, the core curve fitting algorithms are 8+ years in the making and also includes weighting algorithms which are a...

The 4 Parameter Logistic or 4PL is sufficient if you know that your sigmoidal curve is symmetrical around the inflection point. If not, then the 5PL would be a better choice. Here is a post dedicated to the...

Hi, I used my company's http://www.miraibio.com/masterplex-readerfit/curve-fitting-for-plate-readers.html" and used the Best Fit feature (and optimized for root mean square error) to find the best model equation and it appears to fit the 4 Parameter Logistic or 4PL surprisingly well...

I know this post thread is old but my hope is to help others that still have the same question regarding elisa analysis and the 4-parameter logistic equation. The 4-parameter logistic regression model assumes symmetry around the inflection point of the standard curve. On the other hand, the...