Recent content by soundlover

no i updated the initial post with: and the distance between the two coding regions, does it matters?

probably this should be in the chemistry section. my bad

hello all, a friend of mine has built a plasmid to express a recombinant protein in yeast, but she´s having problems in expressing the protein, the level of expressed protein being to low. i do not recall what is the vector used, the promoter is Gal4. after that result, she re-analyzed the...

Hello All I have this issue to discuss in my cellular physiology class: "It is useful to evaluate cellular viability thru invasive methods? " First of all, the concept of "cellular viability" can be understood as the capacity of the cell to multiply, right? what is a invasive...

lo all, i´m having a small prob here -extract the caffein of the tea leaves with CH2Cl2 + NaOH - after the liquid-liquid extraction the organic phase (that contains the caffeine) was evaporated. then i joined some hot "toluen" (C6H5CH3) to the caffeine to occur a cristalization by slow...

lo all,first post here,this seems to be a nice forum:) i´m trying to know a litle more about Immunodetection,how does it work,witch techniques are applied,etc. thanks in advance for any info,responses,links,etc :smile: take care