Well, my value doesn't fit to the straight line on the graph. Thats why I was wondering if I could use the beer lambert law equation to figure out the concentration from the absorbance value I got- or if the value really is too low then I would just have to put zero.
Yes there was some absorbance, but too low to read off the standard graph given to us. Yes we had a blank solution- The spectrophotometer was zeroed against water.
I was going to try and use the Beer Lambert Law equation to try and get an answer. Yes I know- I was just going to try using the Beer Lambert Law equation, but because the straight line on he standard curve didn't go to such low values, will I just need to say that the absorbance was 0, along...
Hi! Sorry I realised its quite hard to understand what I'm talking about. I think I've figured it out though.
For the sodium- so, it was diluted by a factor of 20. so multiplying the concentration of the diluted sample by 20 gives me the concentration in 0.5ml. I can then find out the number...
Apologies for the stupid questions but I have confused myself.
I was separating out blood components in lab- column chromatography- and now we have to calculate Na+ content of each fraction collected (in μmoles) and the protein content of each fraction in mg. Each fraction was 1.5ml.
The Na+...