Enzymes Definition and 36 Threads

I am finding glucanase with highest activity. All the papers I searched with "glucanase characterization" end up having a value called specific activity, with unit u/mg. However, U, according to wikipedia, is the amount of enzyme that catalyses the conversion of one micromole of substrate per...

Hello ! We all now that enzymes lower the activation energy by creating both covalent bonds and week non covalent bonds , in this book , about how the enzyme lower the activation energy using covalent bonds they said : this sentence is not compatible with a basic principle that i learned in...

we assume that we have an exergonic reaction , a substrate going to a product , is the negative ΔG′° the principal reason that the equilibrium favors the product (there is more product than substrate at equilibrium )?, or it is not and that depends on other factors? , thanks .

Hi everyone, I'm curious if anyone knows of a reference book on enzymes that discusses each enzyme's catalytic mechanism(s). I've consulted several books that explain the different categories of enzymatic catalysis but I'm looking more for an encyclopedic reference that explains such...

Pop culture interpretation: Machine learning reveals recipe for building artificial proteins Actual Study: Evolution-based design of chorismate mutase enzymes Using Boltzmann machine learning to design enzymes with evolutionary compatible statistical constraints is possible now. How much...

Transient Receptor Potential cation channels, or TRPs, are special proteins on us which can detect temperature changes or special chemical substances. Like menthol to TRPM8 , or Capsaicin to TRPV1. Is there any current research that are trying to produce these sort of receptors via...

Does the human DNA contain information that could, combined with other data, tell what kinds of chemicals will their digestive system process or not? For instance, there are people that have hereditary fructose intolerance (not to be confused with fructose malabsorption). Is such a lack of an...

Hello I have read this article about why doesn't the stomach digest itself https://www.scientificamerican.com/article/why-dont-our-digestive-ac/ but also during the digestion along the small intestine, there are enzymes. How does the small intestine defend against these enzymes? ( I suppose...

Good job guys I will like to know how to extract the digestive enzymes from the followings: 1.PROTEASE FROM GUAVA,GINGERROOT,PAPAYA AND PINAPLE. 2.AMYLASE FROM BANANA,SWEET POTATO,EGG,SUGAR CANE AND CORN. 3.LIPASE FROM COCONUT, AVOCADO AND CORN. 4.CELLULSE FROM AVOCADO. 5.MALTASE FROM...

1. A substrate molecule may be bound to the active site of an enzyme by all of the following EXCEPT A. Hydrogen Bonds B. Peptide Bonds C. Ionic Bonds D. Van der Waals Interactions E. Hydrophobic Interactions 2. Which of the following components is the most important in determining the 3-D...

Since enzymes have particular temperature on which they are able to catalyze the reaction and increasing the temperature above optimum can damage enzymes then how does poiklotherms survive as they change their body temperature according to environment unlike homoiothems. Also wouldn't their...

Based on the very basic understanding on enzymes I have gained from Year 12 Biology and Chemistry, I don't undesrtand why the enzymes specific shape also helps the reverse reaction. I guess my trouble would be due to the simplified diagrams I've seen from Biology, where only the forward reaction...

Homework Statement Hi just a quick question. I was doing a lab report and this enzyme is supposed to work between the pH of 6.7 and 7. So I had solution of .0125 N and I said OK just subtract this from 7.0 and say that is the new Ph. So for instance 7.0 -0.125 = 6.98 I said OK this is...

I need to preserve frozen food (uncooked) as long as possible. One of the damages caused by long term storage would be enzymes in the food itself. If it is a raw product, i can't exactly heat it up to denature the enzymes. Can i however, use other methods? I was thinking electricity...

I'm new to the subject and i have an intuition that during the time the substrate and the enzyme are together energy is transferred to the substrate (from the enzyme) to break the bonds and to release the substrates energy*. But alas i have been met with opposition to this understanding...

I'm currently doing an EEI with catalase and hydrogen peroxide. I'm just wondering about the effects of increasing the temperature of the catalase and h2o2. For example, if I get 50mL of oxygen produced when both are at 40°C and the reaction occurs for 8 seconds. If I decrease the temperature to...

enzymes are considered inactive at low tempertaures, and denatured at high temps.both these terms have different meanings, with inactive meaning enzyme having "low activation energy"and denatured meaning the enzyme is destroyed. when an enzyme is inactive, it does not lower the activation...

Where can i get LIPL-4

Hello everyone, [PLAIN]http://users.rcn.com/jkimball.ma.ultranet/BiologyPages/R/RestrictionEnzymes.gif 1. Restriction enzymes cut only sugar phospahte bonds right. So in the formation of sticky bonds for example in BamHl, how does the hydrogen bonds between the molecules break. When sugar...

Everything I read says enzymes are organic catalysts and do not supply energy for chemical reactions. My understanding is they speed up reactions by providing alternate pathways that have a lower activation energy. A quiz I took asked "What type of energy do enzymes contribute to chemical...

Homework Statement I have a lab tomorrow and it says it my lab book, and it says to add distilled water to a reaction consisting of the substrate then another one with the enzyme...i don't get it what's the purpose of distilled water...i really don't understand. Thanks.. Homework Equations The...

As in Title, help much appreciated! thanks

is it true that cooperativity is rare in enzymes with tertiary structure and that Glycolysis is inefficient and therefore rarely found in present day organisms i am not sure.. but iwas also wondering that a particular enzyme is likely subject to only a few forms of the regulation we...

I've always wondered how enzymes could tell the difference between too much substrate and too little substrate to initiate allosteric activities, my teacher tells me that its kinda like collision theory in chemical bonding. That would make sense because if there's too much product, then some of...

why would utilizing duo restriction enzymes for cloning be better then just one? ( e.coli to pBluescript II )

enzymes in dehydration synthesis/condensation reaction and hydrolysis?? what is the name of the enzyme that aid dehydration synthesis/condensation reaction and the enzyme that aid hydrolysis? thank you very much

Are there any known enzymes that will fix oxidative dna damage without correcting mismatched bases? Any enzymes that are thought to work like that? thanks

Does anyone know commercial providers of enzymes ?

Dear all ! Does anyone have experience with methanotrophic bacteria ? The background of my question: I am trying to explore a new pathway for the reaction methane to methanol. One can basically think of two approaches: a) chemically via classic catalysts, b) biologically via enzymes or...

Homework Statement Formulate your own definition of what is meant by living organisms. *I also posted the lab which this question is asked on in a thread called "Enzyme Lab." Homework Equations The Attempt at a Solution I don't think that this answer is right but don't know...

Hello, how can large variation between sets of different Ph results be scientifically explained, i am investigating the effects of Ph on enzyme activity, there are small error bars in my results between ph1,2,3,4 &5 however there is larger error bars between ph-6,7,8,9 & 10. there's an...

hello every 1, i have a few questions -i'd like to know what error bars are & how did i plot them on a graph? -can some one please give me a scintific explanation to why small drops of substrate are introduced into a enzyme when trying to find its activity, all i know is that if all the...

Which of the following are true about the following reaction ? O3 + O --> 2 O2 a) The direct reaction breaks down O3 at a slower rate than the same reaction where Cl is used as a catalyst b) When Cl is used as a catalyst, it functions as an intermediate in...

can somebody help me with the following question? What is the role of enzymes inside cells? please help it would be much appreciated :confused:

Hey guys; I've a question. How do enzymes lower the free energy of activation? My thinking is that the enzyme binds, and when it binds it alters the shape of whatever it binds to, and the altered shape is energetically favored to the unaltered shape for the reaction. This is purely...

I'm wondering if, when put into the DNA together, two different restriction enzymes will interfere with each other, and how. Will two different restriction enzymes cut the DNA as they each would separately, since they are specifically formed to react with it and not each other, or would they...