16S rRNA sequence information to identify organisms

Click For Summary
SUMMARY

The discussion focuses on identifying an unknown 16S ribosomal RNA gene sequence using online tools. The user seeks a program that can match the provided sequence to known bacterial species. The recommended tool for this purpose is NCBI's BLAST, which allows users to input sequences and obtain close matches from a comprehensive database. This method is essential for microbiologists and researchers working with bacterial identification.

PREREQUISITES
  • Understanding of 16S ribosomal RNA gene sequences
  • Familiarity with NCBI's BLAST tool
  • Basic knowledge of bacterial taxonomy
  • Experience with sequence alignment concepts
NEXT STEPS
  • Learn how to use NCBI's BLAST for sequence alignment
  • Explore the principles of phylogenetic analysis
  • Research the significance of 16S rRNA in microbial ecology
  • Investigate alternative sequence identification tools like SILVA or RDP
USEFUL FOR

This discussion is beneficial for microbiologists, bioinformaticians, and researchers involved in microbial identification and taxonomy using genetic sequencing techniques.

University
Messages
31
Reaction score
0
Hi All,
I have an unknown 16S ribosomal RNA gene sequence below that I am trying to identify. I know its a bacteria but anyone know of a site/program online that allows you to enter the sequence below and it gives the likely close match?




TAGGGAATCTTCCGCAATGGACGAAAGTCTGACGGA
GCAACGCCGCGTGAACGATGAAGGCCTTCGGGTCGT
AAAGTTCTGTTGTTAGGGAAGAACAAGTATCGGAGTA
ACTGCCGGTACCTTGACGGTACCTAACCAGAAAGCC
ACGGCTAACTACGTGCCAGCAGCCGCGGTAATACGT
AGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAG
CGCGCGCAGGCGGTCCTTTAAGTCTGATGTGAAAGC
CCACGGCTCAACCGTGGAGGGTCATTGGAAACTGGG
GGACTTGAGTACAGAAGAGGAAAGCGGAATTCCACGT
GTAGCGGTGAAATGCGTAGAGATGTGGAGGAACACCA
GTGGCGAAGGCGGCTTTCTGGTCTGTAACTGACGCTG
AGGCGCGAAAGCGTGGGGAGCAAACA
 
Biology news on Phys.org

Similar threads

Mixed Culture 16s rRNA Analysis Q&A
  • · Replies 1 ·
Replies
1
Views
3K
Brave New World of Protein Design
  • · Replies 4 ·
Replies
4
Views
3K
Semi-synthetic organism with an expanded genetic code
  • · Replies 8 ·
Replies
8
Views
5K
RIP Carl Woese, of three-domains fame
  • · Replies 1 ·
Replies
1
Views
4K
Bacterium with a Minimal Genome
  • · Replies 26 ·
Replies
26
Views
10K
Custom Nucleotide Sequence Codons?
  • · Replies 12 ·
Replies
12
Views
5K
New CRISPR-based tool for find-and-replace editing of DNA
  • · Replies 2 ·
Replies
2
Views
5K
Eukaryote high-level evolution - have we succeeded in mapping it?
  • · Replies 2 ·
Replies
2
Views
4K
Synthesis of a re-designed E. coli genome
  • · Replies 3 ·
Replies
3
Views
3K
New Yorker Article about epigenomics
  • · Replies 8 ·
Replies
8
Views
4K