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4 parameter logistic model for fitting PCR data

  1. Mar 7, 2015 #1
    I have a question about the logistic fit they used on this paper: http://online.liebertpub.com/doi/pdf/10.1089/cmb.2005.12.1047. Its PDF page 5, or journal page 1051. They define all the variables and then say x0 and b define the shape of the model. If I am fitting 30 cycles of PCR data how am I suppose to know what to use for x0 and b? -Thanks
     
  2. jcsd
  3. Mar 8, 2015 #2

    mfb

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    2016 Award

    Staff: Mentor

    Use your fit results? Those results should be the unknown constants.
     
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