Well, it's more dramatic with complex amplitudes, but interference effects would show up even if all amplitudes are positive real numbers.
Suppose you do a double slit experiment with positive real amplitudes. A photon can either go through the left slit, with probability p, or the other slit, with probability 1-p. If it goes through the left slit, say that it has a probability of q_L of triggering a particular photon detector. If it goes through the right slit, say that it has a probability of q_R of triggering that detector. Then the amplitude for triggering the detector, when you don't observe which slit it goes through, is:
\psi = \sqrt{p} \sqrt{q_L} + \sqrt{1-p}\sqrt{q_R}
leading to a probability
P = |\psi|^2 = p q_L + (1-p) q_R + 2 \sqrt{p(1-p)q_L q_R}
That last term is the interference term, and it seems nonlocal, in the sense that it depends on details of both paths (and so in picturesque terms, the photon seems to have taken both paths). Without negative numbers, the interference term is always positive, so you don't have the stark pattern of zero-intensity bands that come from cancellations, but you still have a similar appearance of nonlocality.